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[*]Due to encoded by plasmid, MicroRNA sponge can silence target MicroRNA stablely through eukaryotic expression vector and packaged lentivirus particle.

[*]MicroRNA sponge is especially suitable for the functional research of a family of MicroRNAs because of the conserved seed sequences.

[*]MicroRNA sponge is particularly valuable for long-term loss-of-function study both in vitro and in vivo.

[*]Even completely unrelated miRNAs binding sequences can be constructed compatibly within one vector, so MicroRNA sponge could slience multiple miRNAs simultaneously.

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