Réussir vos études
AccueilAccueil  PublicationsPublications  S'enregistrerS'enregistrer  Connexion  
Derniers sujets
Retrouvez-nous sur Facebook

Site partenaire
Plus de likes gratuitement Likeup.fr - Plus de Likes, Followers, Google+, Vues et d'Abonnés
Articles Semilaires

Partagez| .

Native Proteins Background

Voir le sujet précédent Voir le sujet suivant Aller en bas
Recherche personnalisée
MessageSujet: Native Proteins Background Ven 16 Déc 2016 - 7:15

In biochemistry, the native state of a protein is its properly folded and assembled form with operative structure and function. The native state of a protein needs all four levels of biomolecular structure, with secondary to quaternary structure formed by weak interactions along the covalently-bonded backbone. Proteins in their natural state with intact structure that is not altered by heat, chemicals, enzyme reaction, or other denaturants are named "native proteins".
Compared to recombinant protein generated using protein engineering techniques, native protein are obtained from proper organisms and are unaltered by outer factors such as heat, chemicals and enzyme reaction, and retain their naturally folded state. With natural structure and functional activities, native proteins are used in a wide range of applications such as protein functional assays, ELISA kits, Western Blot, Immunoprecipitation, etc.
The native state of a protein is defined as the ensemble of compact conformations, forming under folding conditions in vivo or in vitro. In native state, the protein is stable and active. In biophysics, the native state of a protein is defined by a low amount of entropy with a relatively low intramolecular enthalpy. In native state, all atoms of the protein are kept by mutual interaction in a well-defined geometry. The native state of a protein can be distinguished from a molten globule by distances measured by NMR.
Altering the folding conditions such as increasing temperature, changing buffer pH or adding denaturant can result in a much more complex and heterogeneous ensemble of protein conformations, which are named the denatured states. In addition, different unfolding conditions induce different denatured states. Strong unfolding conditions induce high percentage of secondary structure changes. Proteins in denatured states can be detected with relatively high conformational entropy and intramolecular enthalpy, because of the loss of native interactions.


Sexe : Féminin
Messages Messages : 1
Points Points : 3
Date de naissance Date de naissance : 18/11/1989
Age Age : 28
Emploi Emploi : USA

Revenir en haut Aller en bas
Native Proteins Background
Voir le sujet précédent Voir le sujet suivant Revenir en haut
Page 1 sur 1
Sujets similaires
» Alénor d'Aquitaine native de Belin
» [Background de la Campagne] La légende du Capitaine Crown
» APPROPRIATION CULTURELLE : où est la limite entre promotion des valeurs traditionelles et les choix purement esthétiques ? (coiffes indiennes, bindis, etc)
» Recherche sprite , tiles, background genre futuriste
» Pouvez vous me créé un background?

Permission de ce forum:Vous ne pouvez pas répondre aux sujets dans ce forum
INTIK :: Univers des Biologistes :: Cours de biologie :: Biotechnologies-